Download e-book for iPad: An Engineering Introduction to Biotechnology by J. Patrick Fitch
By J. Patrick Fitch
This instructional can help technical pros in optics verify even if their applied sciences have power software within the existence sciences. It is also necessary as a ''prep class'' for extra unique books on biology and biotechnology, filling the space among basic and high-level approaches.
- easy Biology
- Nucleic Acids because the Blueprint
- Manipulating Nucleic Acids and Proteins
- An built-in technique for organic Discovery
- DNA Sequencing
- Detecting Nucleic Acids
- Protein Structure
- Appendix A: devices and Measures
- Appendix B: Nonscientific Issues
- prompt Reading
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Additional resources for An Engineering Introduction to Biotechnology
Of the gel-based techniques, two methods are most common: linear agarose gel separation and gradient gel separation. Agarose separation is similar to DNA separation; the “friction” of the gel separates 43 44 Chapter 3 Fig. 1. Agarose gel box for electrophoretic separation of negatively charged DNA by size (length). Fig. 2. Radioactively labeled gel comparing DNA size for a family that includes members with and without myotonic dystrophy. As the number of trinucleotide (CTG) repeats increases, the severity of the disease increases.
For example, a genetic “switch” has been demonstrated in the E. coli bacterium (see Gardner, Cantor, and Collins, Nature, vol. 403, pp. 339–342, Jan. 2000). One of the biggest obstacles to gene therapy is that it is almost impossible to regulate a dose. The patient has to be given a large quantity of genes through a modified virus (also known as a virus vector), resulting in a high probability of negative immune response and possible death. Thus, the ability to implement stable regulatory pathways for gene expression is critical for genomic approaches to fully affect human health.
Examples of single gene disorders that are autosomal recessive include Tay-Sachs disease, phenylketonuria (PKU), cystic fibrosis (CF), and sickle cell anemia. Legend Male (XY) AB Female (XX) CD Affected Male Deceased Female AC AD BC BD Heterozygous Male Parents Children Fig. 3. Graphic description of mendelian inheritance of genetic traits A, B, C, and D in progeny. Using the different designations, the reader might develop a family tree for an individual with an autosomal recessive disorder. How might grandparents and siblings appear on the tree?
An Engineering Introduction to Biotechnology by J. Patrick Fitch